OpenSlideUnsupportedFormatError Traceback (most recent call last) If we try to open this png image with OpenSlide, it crashes immediately saying that it is an unsupported file type: We begin with this 2,000 x 2,000 pixel png image: This blog post discusses how to take anyimage and convert it into an OpenSlide compatible WSI, with embedded metadata. Unfortunately, when smaller regions of interest, or new images, are created in tif/png/jpg formats they no longer remain compatible with OpenSlide. Openslide provides a reliable abstraction away from a number of proprietary WSI file-formats, such that a single programmatic interface can be employed to access WSI meta and image data. Many digital pathology tools (e.g., our quality control tool, HistoQC), employ Openslide, a library for reading whole slide images (WSI).
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